BILB 477-01: IMMUNOLOGY LABORATORY LAB EXCERCISE 2: PLURIPOTENT STEM CELL LAB SIMULATION (50 POINTS) INSTRUCTIONS: (1) Wrtte student names t the top of this question shest; (2) Anawer l queationa on separave (tped) sheet and staple to the baok of this question sheet; (8) DUE: 01102/19 LAB SIMULATION QUESTION8: (1) What was the purpose of the pluripotent stem cell lab simulation? (2 points) (2) Discuss all relevant differences between totipotent, pluripotent, and multipotent stem cells. (5 points) PSCs) and induced pluripotent stem cells (iPSCs). (5 points) and iPSCs. Which of these systems would be best utilized in a clinical application? (5 (3) Discuss all relevant differences between embryonic stem cells (pluripotent stem cells; (4) Discuss differences in feeder-dependent and feeder-free systems in the culture of PSCs points) (5) What is the difference between basic research and clinical research? (3 points) (6) Discuss the use of fibroblasts in the culture of PSCs and iPSCs. Which culture system (7) Describe the morphology of PSCs and iPSCs grown in the feeder-dependent culture (8) Why is monitoring the morphology of PSCs and iPSCs critical in the establishment of a new (9) What was the substrate vitronectin (VTN-N) used for in the culturing process? (5 points) (10) Why should we validate a newly established PSC or iPSC cell line? What two utilized these cells and from what origin were they derived? (5 points) system. (5 points) stem cell line? (5 points) makers where probed for in this lab simulation? (5 points) (11) Name the validation method used in this lab simulation, and then describe its theory and application. (5 points)