Question: A Procedure Has Been Devised For Purifying A Particular Enzyme Using Gel Chromatography. In An Effort To Increase The Amount Of Material To Be Handled, You Use A Sample Whose Concentration Of Protein Is Ten Times That Normally Used With This Procedure. The Enzyme Activity Now Elutes Principally In The Void Volume. Explain What Happened.
A procedure has been devised for purifying a particular enzyme using gel chromatography. In an effort to increase the amount of material to be handled, you use a sample whose concentration of protein is ten times that normally used with this procedure. The enzyme activity...