DILUTION PROBLEMS 1. Let’s say a stock culture of E. coli was diluted by serial dilution as indicated below Then, 0.1 ml of suspension was removed from each tube and spread on a plate, and the colonies were counted, producing the following results: TUBE CONTENTS NUMBER OF COLONIES INDIVIDUAL DILUTION TOTAL DILUTION DILUTION FACTOR 1 ml stock+99 ml water Tube 1 TNTC 1 mi tube 1+99 m io 2. Io Tube 2 water IO Tube 3 1 ml tube 2+9 m 153 water Tube 4 1 ml tube 3 + 9ml 17 water Tube 5 1 ml tube 4+ 9 ml What is the CFuimi count for the undilited stook? z. A stook oulture of E. coll wan dilluted by serial dilution as indicated below. Then, tube and spread on a plate, and the 0.1 ml of suspension was removed from each NUMBER OF COLONES!INDIVIDUAL DILUTİON! |DILUTION FACTOR TUBE CONTENTS TOTAL DILUTION Tube 1 1 ml stook+99 m 1 mi tube 1+9m 1 ml tube 2+9ml 1 mi tube 3+9m 1 ml tube 4 +9m Tube 5 water What is the CFU/ml count for the undilutod stock? 3. A stock culture of E. coll was diluted by serial dilution as indicated belaw. Then 0.1 mi of suspension was removed from each tube and spread on a plate, and the colonies were counted. producing the following results: | TUBE CONTENTS | NUMBER OF COLONIES | İNDIVİDUAL DILUTION | TOTAL DILUTION 1 mi stock +9m DILUTION FACTOR 836 Tube 3 water stock? Lab 4 ! Quantitative plate Oount· 41 4. A stock culture of E coli was diluted by serial dilution as indicated below. Either 1.0 ml or 0.1 ml of cells was removed from each tube, mixed with melted ogar, and poured into a plate, and the colonies were counted Use the first table to calculate the dilution factor for each of the four tubes, then TOTAL DILUTION DILUTION FACTOR 6 1 ml stock +99ml Tube 1 Th 1 ml tube 1 +99 ml Tube 2 3 1 ml tube 2 +99m Tube 3 1 ml tube 3 +99 ml Tube 4 AMOUNT ADDED FROM EACH DILUTION TO THE MELTED POURED AGAR DILUTION FACTOR NUMBER OF COLONIES Tube 1 Tube 2 Tube 2 Tube 3 Tube 3 Tube 4 1.0 ml trom tube 2 0.1 ml from tube 2 1.0 ml from tube 3 0.1 mi from tube 3 1 0 ml from tube 4 TNTC 1,243 231 What is the CFU/mi count for the undiluted stoak?