Emay-1 (ts points)nisnuthless fedlow, married Borte. However, instead of their son Kubalind lie his mother, was ll-min Gengs (am) who shared her father’s and grandmother’s (murderous) traits. These traits are linked to potymorphiams in the gene Mayhem. Hint (Use PCReSequencing data to identily the RFLP and the basis of the disesse) Major 1) identifly the location & mutation in the Maxtem gene in Genghis 2) For LmG, predict RFLPs on Southem Bots and effects on mRNA Protein. 3) Upon his dioce, Genghis approached your company MlecilorMotch moten’ (мамиО looking for. new spouse, oe of your (recently divorced) dients, Ms. A-L, seemed interested in Genghis Based on her Molecular Profile’ (Southern, Northern, Westem Blots), what is the likellhood of their sons exthibiting the Ruthlessymurderous’ trat How can Alkhtar share her mum’s genotype, but not the trait? Why is this also seen with Borte & her mumi S) What can you infer about the location& nature of the mutation in the X2 allele (Borte & her mum) 6) Design POR+REPL based dagnostic for the X4 alele i, M. Alicannot be based on DNA sequencing). 7) Minoc. How would you study the mouse homologue, wee-Beastie (be brief Eooft EcoR1 Mayhem , TS PCR-Primers binding stes & direction (5-+3 Temulen A Names &associations ae Scional Only or L-miss-Genghls Kba Southern: E-EcoR1, puPsti, SaSmat. XsXho1 Ms. A-J Temulen Genghis LMG Kb 10 Ms. AJ Temlen Borte Genghis LmG Northern GAPOH -50% . D 100% -50% -s1% PCR & Sequencing P2+P3 P4+PS P3+P4 ATCeec.ca Ge | 24.AAR. A9TR.e ax.c.cs.s.9.eWXRAARAA