Essay-1 С15 pointsJ: Genghis. a ณ thie”fellow, married Berte. However, instead of their son Kublai (kind Ike his mother), t wasL8-miss- Gengis (LmG) who shared her father’s and grandmother’s Imurderous) traits. These traits are linked to polymorphisms in the gene Mayhem Hint (Use PCR-Sequencing data to iderefy the RFLP th bass of the disease). 1) Identify the location& mutation in the Mzuhem gene in Genghis 2) For LnG, predict RFLPs on Southern Blots and effects on mRNA+Protein. 3) Upon his divorce, Genghis approached your company Moleculor-Motchmakers (M&M-LLC) looking for a new spouse. One of your (recently divorced) clients, Ms. A-J, seemed interested in Genghis. Based on her Molecular Profle’ (Southern Northern, Western Blots), what is the likelhood of their sons exhibiting the 4) How can AkHtar share her mum’s genotype, but not the trait? Why is this also seen with Borte&her mum 5) What can you infer about the location &nature of the mutation in the X2 allele (Borte& her mum 6) Design PCR RFPL based diagnostic for the X4 allele in Ms. A-l (cannot be based on DNA-sequencing). 7) Minor: How would you study the mouse homologue, wee-Beastie (be briej? Mayhem O Kb) Southern: E EooR1, P-Pst1, S Smat, X-Xhot Ms. A-J Borte Genghis L-m-G 10 Ms. AJTemulen GenghisL-mG Northerm GAPO Westerrn PCR & Sequencing TCCCCCaGs