Essay-1(15 points): Genghis, a puthlass tellaw, married Börte. However, instead of their san Kublai (kind like his mother), it was Lil-miss Gengis (LmG)whosharedher fathers and grandmother’s (murderous) traits. These traits are linked to polymorphisms in the gene Mayhem. Hint (Use PCR+Sequencing data to identify the RFLP and the basis of the disease. Maiar 1) kdentify the location & mutation in the Mawhemgene in Genghis. 2) For LmG, predict RFLPs an Southern Blots and effects on mRNA+Protein. 3) Upon his divorce, Genghis approached your company Malecular-Matchmakers’ (M&M-LIC) looking for a newspouse. One of your 令ecently divorced) clients, Ms. AJ, seemed interested in Genghis. Based on her Molecular profile (Southern, Northern, Western Blots), what is the likelihood of their sans exhibiting the Ruthless/murderous trait. 4) How can Akhtar share her mums genatype, but not the trait? Why is this alsoseen with Borte&her mum? 5) What can you infer about the location & nature of the mutation in the X2 allele (Borte &her mum). 6) Design a PCR+RFPL baved diagnostic far the X4 allele in Ms.A-J (cannot be based on DNA-sequencingl 7) Minar: How would youstudy the mouse homologue, wee-Beastie (be brief? Mavhem Xht EcoR O Kb) (3 Kb) 4 TS PCR-Primers binding |P1→ sees & direction (5+3)| P3→ P6 Ms.A Borte Temüge Temulen Akhtar Genghis Behte ORutless Kind Names & associations are fictional Only for education entertainment Lil-miss-Genghis ublai Southern: E-EooR1, P-Pst1, S-Sma1, X-Xho Ms. A-J Temulen Borte Genghis L-m-G P +S 10 MS.AJTemulen Borte GenghisLm-G Northern Mayhem GAPDH 00% . -50% Western ー50% ..100% -50% s1% PCR&Sequencing P2+P3 CATG