Homework 3: Molecular Techniques due Monday 1/30/19 10 points name 1. (2 points) Match the following with its role a. Ori b. Promoter c. Ligase d. Primer Enzyme that is needed to seal Okazaki fragments together. This enzyme is also important in recombinant DNA technology in that it seals pieces of DNA together Sequence in the DNA that allows for the opening of the helix and entry of the DNA polymerase A segment of nucleic acid that is needed in order to begin the DNA synthesis process. In living cells it is a piece of RNA. When performing DNA synthesis in the lab it is a piece of single stranded DNA. Sequence in the DNA that is important in regulating the expression of a gene. This is the only component listed that is used in the transcription process 2. (3 points) Regarding the Translation process a. (0.5 point) In what order do the following components add in to form the initiation complex? use numbers I through 3 _Met-tRNA Met _mRNA Large Subunit b. (1 point) What are the three steps in translation clongation? In what order do they occur? c. (1.5 points) Describe the process of termination. What additional component is involved, where does it bind within the ribosome and what happens once it binds?