17 Aug Question: In This Experiment You Are Enumerating Antibody Producing Cells And You Are Characterizing The Class Of Antibody Produced By Mice Mounting Either A Primary Or A Secondary Immune Responses To Sheep Red Blood Cells You Are Testing 1/1000th Of The Spleen From These Mice With The Following Results: Table 1. Groups Primary Secondary 1. Spleen Cells Plus …
In this experiment you are enumerating antibody producing cells and you are characterizing the class of antibody produced by mice mounting either a primary or a secondary immune responses to Sheep red blood cells
You are testing 1/1000th of the spleen from these mice with the following results:
Table 1.
| groups | primary | secondary |
| 1. Spleen cells plus Complement | 240 | 25 |
| 2. As in 1, plus Goat anti mouse IgM Antibody | 10 | 0 |
| 3. As in 2, plus Goat anti anti mouse IgG | 75 | 920 |
| 4. As in 2, plus Goat anti anti mouse IgG1 | 34 | 270 |
| 5. As in 2, plus Goat anti mouse IgG2a | 39 | 200 |
| 6. As in 2, plus Goat anti mouse IgG1 and antiIgG2a | 77 | 490 |
I. Calculate the number and type of PFCs per spleen in Groups 1 to 5 for both 1ary and 2ary responses.
II. What characteristic difference do these results illustrate between primary and secondary immune responses mounted by these mice?
III. What can you conclude about the differences in the subclasses of IgG produced in the primary and secondary immune responses? Explain of how you arrived in to your conclusion?
IV. Experimental evidence, primarily obtained from data employing the Jerne plaque assay, has verified that IgG1 and IgG2a antibodies are made by distinct B cells. Can the above data confirm the above principle? Reason your answer
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