le that cells exhibit great variability in terms of their shape, sizes and nu organelles. The relationship between shape and function is called the principle of- Let’s analyze some limitations of light microscopy: – Red blood cells looked round, but in reality they are biconcave. What method of microscopy would allow you to see their real biconcave shape? -Microvilli on enterocytes looked small and fuzzy. Compare what you observed with picture 2.7 p. 24 in your Histology Atlas. What is the method of microscopy used in that picture? How would you explain the inability to observe all the organelles in some cells such as muscle cells? Light microscopy has relatively low Which alternative methods of microscopy (name at least 2) would you use to be able to visualize more organelles? Let’s analyze the need for different types of stains – When you looked at the spinal cord, the Cresyl violet stain showed the ER (Nissl bodies) of neurons in the gray matter, however in the white matter the tissue looked colorless. The white matter contains numerous myelinated axons. If you wanted to enhance the myelin in those axons what kind of stain would you use instead? -Goblet cells looked “transparent” (although they contain a large number of carbohydrates). Which alternative stain would you use to visualize the carbohydrates? – What is the advantage of using Wright stain when visualizing blood cells? Wright stain enhances the stain. stain. (lysosomes) in white blood cells