17 Aug Question: Need Help With These Genetics Questions Please!! 1. A Student Created A Mutation In The Bli-4 Gene And Introduced This Gene Sequence Into C. Elegans. The Mutation In This Gene Disrupts The Structure Of The Adult-stage Cuticle, Causing Fluid Filled Separations Of The Cuticle Layers. The Student Then Engineered A Bacteria To Carry SiRNA Targeting The …
Need help with these genetics questions please!!
1. A student created a mutation in the bli-4 gene and introduced this gene sequence into C. elegans. The mutation in this gene disrupts the structure of the adult-stage cuticle, causing fluid filled separations of the cuticle layers. The student then engineered a bacteria to carry siRNA targeting the bli-4 gene in C. elegans, and fed the bacteria to the worms. After a week, the student noticed that instead of seeing adult worms with blisters, most adult worms were paralyzed. Suggest some reasons why he did not see the blister mutation. (3 marks)
2. In a classical genetic analysis, scientists create mutations within or surrounding cloned gene sequences. Then, they introduce these mutated gene sequences into cell cultures or model organisms and examine the phenotypes. Geneticists are now adding RNA-induced gene silencing to their gene analysis toolbox. Describe how you would use RNA-induced gene silencing to study the role of the Drosophila tra gene in sex determination. Next, describe how you would study tra function, using a classical genetic analysis. What are the advantages and limitations of each research approach? [Klug, W. S., Cummings, M. R., Spencer, C. A., & Palladino, M. A. (2015). Concepts of Genetics (11th ed.). Pearson.] (4 marks)
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