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17 Aug Question: Please Help Me Construct This Graph!!! Data Is Provided. The Plot You Are About To Construct Is Called A Standard Population Curve.

Posted at 18:33h in Science by
4. The plot you are about to construct is called a standard population curve because once you know how many CFUs/mL relate to a OD60 0 value, you can use the graph (or the resulting equation Works of the line) to determine the population size of any E. colí sample by measuring the OD600 of an unknown culture in a spectrophotometer. a) Use Excel to plot the OD600 data (Which axis? Is this the dependent or independent variable?) versus the calculated CFU/mL for each culture dilution (Which axis? Is this the dependent or independent variable?). Use a line of best fit to connect the five data points Determine and report the equation of the best fit line. Aveage Absoroance 00.00 O 1:10elo 1608,000 |-0.087 1:10e4、 loo, 800,0001-0.086 1008,000,0000.08 100,080,000,0p 0.098 0800 0 0,000,b 0.150

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