Essay-1 (15 points): Genghis, a ruthless fellow, married Börte. However, instead of their son Kublai (kind like his mother), it was Lil-miss- Gengis (LmG) who shared her father’s and grandmother’s (murderous) traits. These traits are linked to polymorphisms in the gene Mayhem. Hint (Use PCR+Sequencing data to identify the RFLP and the basis of the disease). 1) Identify the location & mutation in the Mayhem gene in Genghis. 2) For LmG, predict RFLPs on Southern Blots and effects on mRNA+Protein. 3) Upon his divorce, Genghis approached your company ‘Molecular-Matchmakers’ (M&M-LLC) looking for a new spouse. One of your recently divorced) clients, Ms. A-J, seemed interested in Genghis. Based on her ‘Molecular Profile’ (Southern, Northern, Western Blots), what is the likelihood of their sons exhibiting the ‘Ruthless/murderous’ trait 4) How can Akhtar share her mum’s genotype, but not the trait? Why is this also seen with Borte & her mum? 5) What can you infer about the location & nature of the mutation in the X2 allele (Borte & her mum) 6) Design a PCR+RFPL based diagnostic for the X4 allele in Ms. A-J (cannot be based on DNA-sequencing) 7) Minor: How would you study the mouse homologue, wee-Beastie (be brief)? EcoRI Smal Pst1 Mayhem溜01) (10Kb) Mayhem (BKb) (10 Kb) 0 Kb) (3 Kb) (4 Kb) TSS Exon-1 PCR-Primers bindingP1 sites & direction (5->3′) P3-* P6 P4 Pa Ms. AJ Borte Temüge Temulen Akhtar Genghis Behter ORuthless OKind Names & associations are fictional Only for education+entertainment. Lil-miss-Genghis Southern: E-EcoR1, P Pst1, S-Sma1, X-Xho1 Ms. A-J Temulen Borte Genghis L-m-G +P +S +P +S +P +9S 9 8 7 5 2 Ms. A-J Temulern Borte Genghis L-m-G -100% Mayhem GAPDH -50% -50% . 50% Northern Western -50% ..100% -50% PCR & Sequencing P2+P3 P4+P5 G CAT G AACAAGT G C GCATGAACA