Chat with us, powered by LiveChat Question: What Does "The PCR Digestion Is A 4X Over-digestion" Mean And Why We Use It ? And How Many Microliters Should I Use For Ddh2o And PCR Product ? | Wridemy

Question: What Does “The PCR Digestion Is A 4X Over-digestion” Mean And Why We Use It ? And How Many Microliters Should I Use For Ddh2o And PCR Product ?

Log in to Canvas-Canvas Supp: D MBB-308-Spring-2019-Lecture,4 e Get Homework Help With Cheg- + x × X С File l file:///C:/Users/Haya/Downloads/MBB-308-Spring-2019-Lecture-4.pdf Apps D civiliumyu.com/uplc-Core Courses IChen irst Year Engineerin D 2015-2016 Calenda diol uli D Materials #Gjpg OSAB TERA Video So O How to Make Cream » 2126 Double digest of PCR product and vector Know exactly what you are going to pipette! I’m showing the plasmic digestion below. The PCR digestion is a 4X over-digestion (recipe not shown). You should understand why. Think about the Moles of DNA being digested for your answer. ddH20 10X NEB Cutsmart buffer (no BSA) (400 ng of pJ014 plasmid or PCR product) EcoR I (get the unit concentration in lab) Xho I (get the unit concentration in lab) Total volume 35-X ul 4.0 ul 0.5 ul 0.5 ul 40.0 ul 10-27 PM ESERE)- %2 @ ,”al 2019. 01-27

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