17 Aug Question: Which Protein Elutes First From The CM-sepharose Column, And Which Elutes Second? Briefly Explain The Reasons That The Proteins Elute In This Order. Helpful Information Is Available On The ProteinParam Application On The ExPASy Web Site (Google Protparam). The UniProtKB Accession Numbers For Horse Myoglobin And Horse Cytochrome C Are P68082 And P00004, …
Which protein elutes first from the CM-sepharose column, and which elutes second? Briefly explain the reasons that the proteins elute in this order. Helpful information is available on the ProteinParam application on the ExPASy web site (Google protparam). The UniProtKB accession numbers for horse myoglobin and horse cytochrome c are P68082 and P00004, respectively (when you are asked for selection of endpoints, choose the top choice, which will be CHAIN)
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