2c. Using the “Trend Line function, do a linear regression on each set of points. Check the Display equation on chart” box. Write the slopes of each linear regression equation for each temperature in the chart below. In each case, the slope is equal to the rate of enzyme activity measured as the change in absorbance per minute (AA4so/min) Enzyme Activity (Assemin) 10 12 2d. Write your conclusion about the effect of pH on lactase reaction rate. Do the data support your prediction? If so, explain how the data supports your prediction. If not, explain what you think may have occurred. What is the optimum pH for lactase (based on your data)? Activity 3: Effect of Temperature on Enzyme Activity Tem Enzyme Activity (AAsslmin) 0 25 50 80 3d. Write your conclusion about the effect of temperature on lactase reaction rate. Do the data support your prediction? If so, explain how the data support your prediction. If not, explain what you think may have occurred. What is the optimum temperature for lactase (based on your data)? Activity 44 4a. Record your experimental data in the table below Absorbance at 450 nm (Aa of-39 lul of64 63 μ1 of 64 125 μ. of 64 (min) ul of mM ONPG 1.0 O. 4.1411 2243し2.141 10223 2.0 4b. On separate paper, plot the data (Time (X) vs. Absorbance (), using 4c. Using the Trend Line function, do a linear regression Check the on each set of points. Display equation on chart” box. Write the slopes of each linear regression equation each temperature in the chart below. In each case, the slope is equal to the rate enzyme activity measured as the change in absorbance per minute (AAsolmin). of ONPG Enzyme Activity (AAss/min) 0㎕Of 64 mM ONPG 20 μ1。f 64 mM ONPG 39 ul of 64 mM ONPG 63 ul of 64 mM ONPG UE 092X 125 ul of 64 mM ONPG 4d. Write your conclusion about the effect of substrate concentration on lactase reaction rate. Do the data support your prediction? If so, explain how the data support your prediction. If not, explain what you think may have occurred. Did you observe enzyme saturation? If so, explain how you can tell. If not, explain why not Activity 5: Effect of Inhibitors on Enzyme Activity Record your experimental data in the table below. Absorbance at 450 nm (A450 1000 ONPG + No ONPG+ No ONPG 1000 и ONPG 50 ONPG 1000 ONPG + 500 (min) -500- T InhibiltorInhibitorXInhibitorx Inhibitor Y Inhibitor Y 0.5 1.5 2.0 300.2131 0 178 10.005 01ooo5 l0. 0olo 5b. On separate paper, plot the data (Time (0) vs. Absorbance ()), using Excel. 5c. Using the “Trend Line function, do a linear regression on each set of points. Check the Display equation on chart” box. Write the slopes of each linear regression equation for each temperature in the chart below. In each case, the slope is equal to the rate of enzyme activity measured as the change in absorbance per minute(AAssolmin). Treatment Enzyme Activity (AA4so/min) 500 l ONPG+ No Inhibitor 1000 ul ONPG +No Inhibitor 500 μ1 ONPG + Inhibitor X 1000 μ1ONPG +Inhibitor X 500 μ1 ONPG + Inhibitor Y 1000 ul ONPG+Inhibitor Y Now, use the data from the above table to plot a bar graph showing reaction rate (enzyme activity) at each of the different treatments. You may need to consult with your instructor on how to do this. 5d. Se. What is your conclusion about Solution X as an inhibitor? Include whether you think it is a competitive or non-competitive inhibitor (or neither) and why. 5f. What is your conclusion about Solution Y as an inhibitor? Include whether you think it is a competitive or non-competitive inhibitor (or neither) and why What was the purpose of the tube that just had a high concentration of inhibitors? 5g. ONPG, but no