Chat with us, powered by LiveChat Question: . Consider The Five Proteins With The Properties Shown In The Table Below. Answer The Questions And JUSTIFY Your Answers (20 Points). Protein Subunit Mass 10,000 15,000 20,000 25,000 30,000 Full Protein Mass Isoelectric Point (pl) 40,000 120,000 20,000 75,000 8.1 5.3 7.2 4.5 6.2 A. Which Protein Would Elute Last From Size-exclusion (otherwise Known ... | Wridemy

18 Aug Question: . Consider The Five Proteins With The Properties Shown In The Table Below. Answer The Questions And JUSTIFY Your Answers (20 Points). Protein Subunit Mass 10,000 15,000 20,000 25,000 30,000 Full Protein Mass Isoelectric Point (pl) 40,000 120,000 20,000 75,000 8.1 5.3 7.2 4.5 6.2 A. Which Protein Would Elute Last From Size-exclusion (otherwise Known …

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. Consider the five proteins with the properties shown in the table below. Answer the questions and JUSTIFY your answers (20 points). Protein Subunit Mass 10,000 15,000 20,000 25,000 30,000 Full Protein Mass Isoelectric Point (pl) 40,000 120,000 20,000 75,000 8.1 5.3 7.2 4.5 6.2 a. Which protein would elute last from size-exclusion (otherwise known as gel filtration) chromatography? (5 points) b. Protein A binds to Protein B via strong interaction. You mix equimolar amounts of protein A and B into a tube, then load that into lane 2 of an SDS-PAGE gel. In lanes 3-7 of the gel you load purified individual proteins A, B, C, D, E (one protein for each lane). In lane 1 of the gel you load a molecular marker standard that shows bands at 10 kDa, 20 kDa, 30 kDa, and 40 kDa. Sketch the SDS-PAGE gel. (5 points) c. Which protein would elute (come out of the column) last from an ANION-exchange column at pH 6.5? (5 points) d. What is the subunit composition of protein D? (5 points)

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