Chat with us, powered by LiveChat Question: E: Flip Up The Door On The Machine's Left And Make Sure The Cuvette Chamber Is Empty. NEVER Power Up With A Cuvette In The Chamber Turn On (switch On Back Next To Plug). After A Systems Check The Time And Date Will Be Display, The Spectrophotometer Is Now Ready For Use. Press The Button (See Figure 3-3). Press < 1> And The < Enter> Enter Wavelength ... | Wridemy

18 Aug Question: E: Flip Up The Door On The Machine’s Left And Make Sure The Cuvette Chamber Is Empty. NEVER Power Up With A Cuvette In The Chamber Turn On (switch On Back Next To Plug). After A Systems Check The Time And Date Will Be Display, The Spectrophotometer Is Now Ready For Use. Press The Button (See Figure 3-3). Press < 1> And The < Enter> Enter Wavelength …

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E: Flip up the door on the machine’s left and make sure the cuvette chamber is empty. NEVER power up with a cuvette in the chamber Turn on (switch on back next to plug). After a systems check the time and date will be display, The spectrophotometer is now ready for use. Press the <>button (See Figure 3-3). Press < 1> and the < Enter> Enter wavelength (550 nm) by pressing <5 5><0> on the key pad and press Enter > Since you do not want a background reading, press Enter> Use a Kimwipes to make sure outside of the cuvette is clean and dry. Insert ‘Blank into the cuvette chamber (clear side towards you) and press the green< Read Blank > pad. . Press right arrow. TABLE 3-1. Dilution of 1.0 mM methylene blue stock solution to make 3 ml working solutions. Final Volume 3 ml per sample Initial Stock Conc. -1.0 mM Absorbance Reading (Asso) Tubel Vol. of Water to add (ml) Vol. of Stock Cuvette No | Conc. (μΜ) | to Your Data Class Average Ba 10 30 50 70 80 90 100 U Unknown 3 ml of Unknown a. Blank, which contains no methylene blue, buf water only it doesn’t mean an empty cuvette. Exercise 1. Dilution of Solutions and Spectrophotometry Unit 3: Page 5

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