Chat with us, powered by LiveChat Question: Hi I Have A Lab Where I Was Introduced To Exctinction Coefficients! Can Someone Please Help Me Find It Given The Data? I Need To Find E. | Wridemy

Question: Hi I Have A Lab Where I Was Introduced To Exctinction Coefficients! Can Someone Please Help Me Find It Given The Data? I Need To Find E.

2. After 5 minutes have elapsed, invert the tubes three times again. Use tube 1 to zero out the spectrophotometer at 595 nm, the wavelength of max. Absorbance of the Bradford/Protein complex. Next, measure the absorbances of tubes 2-5 and record in the table below Tube # Vol. BSA StockVol. Distilled Vol. Bradford Soln. (ml) Final BSA Absorbance at Water (ml) Reagent (m Concentration 595 nm 0.25 10 0.05 0.1 0.15 0.25 0.2 0. 15 0. 242 0. 336 0. 472 0.15 20 4 0.1 30 50 3. Prepare a graph (on the next page) of your results showing the standard curve. From the standard curve, determine the slope of the line, which is the extinction coefficient for the Bradford-protein complex (the product of the reaction). The slope of the line (extinction coefficient) will be used to determine the protein concentration of all our samples and fractions Your determined extinction coefficient of BSA (include units!) o Absorbance vs. Concentration 10 20 30 50 0.115 0242 0.336 0.472 ● Absorbance -9A9EGrx+00343r-a979 0.5 0.4 03 0.2 0.1 10 20 30 50

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