In-class Math Worksheet 2, BS171, Spring 2019 You carry out the following serial dilution as depicted in the image, and 24 hours after plating you count the indicated number of colonies on the agar plate. Use the information from the image to answer questions 11-17. In this problem, you start from a colony count and work backwards through the dilution scheme to calculate the concentration in the original culture tube. Remember, cell concentration is always reported as cells/ml. Syl 50u 00ul =Vi a. b. C. E. coll culture 50μ1 42 colonies 11. (1.5 pts) What is the concentration of E. coli in Tube c? 12. (1 pt) For the dilution used to make Tube c from tube b, what are Vi and Vf? Be sure to indicate which number is each variable. 13. (1.5 pts) What is the concentration of E. coli in Tube b (concentration of stock, Ci)? 14. (1 pt) For the dilution used to make Tube b from tube a, what are Vi and Vf? Be sure to indicate which number is each variable 15. (1.5 pts) What is the concentration of E. coli in Tube a? 16. (1 pt) For the dilution used to make Tube a from the E.coli culture, what are Vi and Vf? Be sure to indicate which number is each variable. 17. (1.5 pts) What is the concentration of the original E. coli culture?