the RNA is synthesized in the 3′ to 5 direction. Question 14 0/ 1 point Transcription involves H-bonding (base-pairing) between the nucleotides of the DNA template and the RNA transcript. ends when RNA polymerase reaches the DNA sequence called the terminator. requires the assembly of a large number of proteins, including RNA polymerase, at the promoter. begins when RNA polymerase and proteins called transcription factors bind to a region of the DNA called the promoter. random 3.4 Question 15 0 1 point What is the difference between a primary RNA transcript and an mRNA (messenger RNA)? Pieces of non-coding sequences, called introns, must be removed from eukaryotic primary transcripts: RNA splicing. In prokaryotes, they are one in the same. In eukaryotes, the primary transcript must be extensively modified– including the addition of a 5′ cap and a 3′ poly(A) tail. In prokaryotes only, the mRNA can include the “recipes” for multiple proteins. Attempt Score: 12/15 Question 12 0 1 point RNA polymerase is catalyzing the formation of a phosphodiester bond; it is building a polymer. This is an endergonic (energy- requiring) reaction. Where does the energy come from? It comes from an exergonic (energy-releasing) reaction. ATP has to be added to every one of these reactions Energy is captured when two of the phosphates are cleaved from the in-coming nucleoside triphosphate Energy is released when the hydrogen bonds between the nucleotides is cleaved