18 Aug Question: Ximena Performed A Bradford Assay After Purifying Lactate Dehydrogenase From Cow Heart. She Prepared A Standard Curve Using Pure Bovine Serum Albumin (BSA) So That She Could Determine The Unknown Concentration Of Her Purified Protein. She Prepared The Serial Dilutions And Collected Absorbance Values (below) At λ = 595nm. The Dashed Line Is The EXPECTED …
Ximena performed a Bradford assay after purifying lactate dehydrogenase from cow heart. She prepared a standard curve using pure bovine serum albumin (BSA) so that she could determine the unknown concentration of her purified protein. She prepared the serial dilutions and collected absorbance values (below) at λ = 595nm. The dashed line is the EXPECTED linear line if the dilutions were perfect. Her actual (experimental) standard curve is the slightly curved fully black line below. Her trendline was calculated as R2 = 0.9423. Discuss below whether this is a good R2 value or not and explain your answer. Should Ximena use this standard curve to confidently determine the concentration of her protein, why or why not? Please ignore the red lines below (they are not part of this question.)
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