You want to A. A self cross 1S. A test cross A dihybrid cross D. A three point cross E. None of the above Part II. 8. You are given two different media synthesis, but your You re-label the tubes A, B, and C and do test the growth of dhe thnee as in minimal media. ent yeast mutants that are unable to gre on minimal medio, bos can yge on ted with arginine. You assume r tubes containing arginine sup gx mised up Minimal Media Mutant 1 Nogrowth Mutant 2 1 No growth No growthGrowth No gowth No growth A, B or C in ondet, and labeling which snep A. Write out the biochemical pathway, putting in the pathway each 12 points) 8 2d B. If you make a mutant yeast cell, that is homozygous for both the mutations 1 and 2, would you expect it to be able to grow on compound C? Explain your answer (5 points) . Four differen is t v of the markers, and label the distance (Gased arg (15 min) thy (21 min) 32win the os aisy Hfr #2 mal (10 min) met (17 min thi (22 min) thr 33 i rp 11 iay Hfr #3 phe(6 min) his(1i min) bio(33 min) st4o miny hrs winy iss s Hfr #4 his (1 10. You have a virus with the genotype all- Cas len- crossed with a wild type vinu The progeny from this cross give the following results: 364 365 rnll- Cas- len- 87 rnll- Cas- + 84 +len- 47 rnll-++ 44 + Cas- len- + Cas-+ rnll- +len- a) what are the linkage distances between these genes (8 points)? odt 100こ118CM b) Determine the linkage order for the three genes (4 points) What is the coefr :3:3 .S 12 11. Nelson has “short fingers” and his father has short fingers. Nelson’s patea paternal grandfather (his father’s fatheit, who is now deceased, had normal maternal grandparents (his mother’s tingers, and they have a son námed George, who has normal Gngers Georgg has two younger sisters, one with short fingers and one with normat s two younger sistets have normal fingers Neson’s moaber has pormal He has two oldet sisters who ate identical ewns, and borh ngss oth of ldlon marnes Linda, who has normal A. Using standard symbols and labels, draw a pedigree illustrating the inheritance of Nelson’s family (19 points) t shont fingers in C. If Nelson and Linda have another child, what is the probability (based on your answer in part b) tha his child will be a son with short finger? (5 points). B. What is the most likely mode of inheritance for short fingers in this family (10 points)? 12. The following rwo genotypes are crossed Aa Bb Ce dd Ee x Aa bb Ce Dd Ee What vwill be the proportion of the following gsnotypes among the progeny of this cros(points each) a. Heterozygous for all five genes 13. The recessive alleles b (black body instead of gray body), pr (purple eyes instead of wildtype red cyes) and vg (omall vestigial wings instead of wildtype normal wings) are all on chromosome 2. Females that were heterozygous b’b-pr’pr- vg’vg- were mated to a male which was bomozygous mutant for all three genes. The following progeny phenotypes were obtained from 1005 flies: Phenotype Number of Flies 412 Gray body, red eyes, normal wings Gray body, red eyes, vestigial wings 61 b Gry body, purple eyes, pormal wings Gray body, purple cyes, vestigial wings Black body, red cyes, long wings 30 28 Black body, red eyes, vestigial wings Black body, purple cyes, long wings 60 Draw a genetic map showing the order of the genes and the distance (20 points) Yloo- loos 1D34 ch Oos