Chat with us, powered by LiveChat Question: You Are Interested In Studying Gene A. Your Boss Gives You The Plasmid Vector Pictured Below For Use In Cloning Gene A. 1. What Does Your Boss Mean By 'clone' In This Sense? How Would Bacterial Cells Be Involved? 2. Explain The Purpose Of The Regions Labeled "ori" And "ampR" In Your Vector 3. For Each Of The 6 Unique Restriction Enzymes Represented ... | Wridemy

17 Aug Question: You Are Interested In Studying Gene A. Your Boss Gives You The Plasmid Vector Pictured Below For Use In Cloning Gene A. 1. What Does Your Boss Mean By ‘clone’ In This Sense? How Would Bacterial Cells Be Involved? 2. Explain The Purpose Of The Regions Labeled “ori” And “ampR” In Your Vector 3. For Each Of The 6 Unique Restriction Enzymes Represented …

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You are interested in studying Gene A. Your boss gives you the plasmid vector pictured below for use in cloning gene A. 1. What does your boss mean by ‘clone’ in this sense? How would bacterial cells be involved? 2. Explain the purpose of the regions labeled “ori” and “ampR” in your vector 3. For each of the 6 unique restriction enzymes represented by recognition sites in the vector and the gene of interest below, state whether or not you could use that restriction enzyme to clone Gene A into the vector, and explain why you could or could not use that enzyme. It’s okay if you have a question that prevents you from making a decision about a particular enzyme. State the question in lieu of an explanation 4. From your answers above, state a simple set of ‘rules’ to follow for choosing the correct restriction enzyme for cloning a gene into a vector. 5. If there was a Xhol site within the ori of the vector, and a second Xhol site upstream (to the left of) the promoter of Gene A, would you be able to use Xhol to clone Gene A? Why or why not? Vector Nde I EcoR I Sal I (4kb) or BamH I BamH I Gene of Interest EcoR I Promoter Kpn I Kpn BamHI Gene A

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