The development of the immune response upon immunization is a dynamic process. In this experiment you are enumerating antibody producing cells and you are characterizing the class of antibody produced by mice mounting either primary or secondary immune responses to SRBCs. You are testing 1/1000 th of a spleen from these mice with the following results: see Table 1. Table 1. No of P.F.Cs Groups: 1ary 2ary 1. Spleen cells plus C’ 220 20 2. As in 1, plus Goat anti mouse IgM Ab 5 0 3. As in 2, plus Goat anti anti mouse IgG 74 870 4. As in 2, plus Goat anti anti mouse IgG1 38 250 5. As in 2, plus Goat anti mouse IgG2a 33 220 6. As in 2, plus Goat anti mouse IgG1 and antiIgG2a 75 490 I. Calculate the number and type (Direct, Indirect or inhibition of direct) of PFCs per spleen in Groups 1 to 5 For both 1ary and 2ary responses. II. What characteristic difference do these results illustrate between primary and secondary immune responses mounted by these mice? III. What can you conclude about the differences in the subclasses of IgG produced in the primary and secondary immune responses? Explain of how you arrived in to your conclusion? IV. Experimental evidence, primarily obtained from data employing the Jerne plaque assay, has verified that IgG1 and IgG2a antibodies are made by distinct B cells. Can the above data confirm the above principle? Reason your answe