Essay-1 (15 paints): Genghis, a n-thlessfellow, married Brte. However, instead of their son Kublai (kind like his mother), t was Lilmiss- Gengis [mG) who shared her father’s and grandmother’s (murderous) braits. These traits are linked to polymorphisms in the gene Mayhem Hint (Use PCR-Sequencing data to ide nt fy the RFLP and the bass of the disease). 1) Identity the location& mutation in the Maybem gene in Genghis 2) For LmG, predit RFLPs on Southem Blots and effects on RNA+Protein. 3) Upon his divorce, Genghis approached your company Molecular Mlotchnokers. (NSM-uca looking fora new spouse. oe of your (recently dvorced) clients, Ms. A-l, seemed interested in Genghis. Based on her Molecular Profile’ (Southern, Northern, Western Blots), what is the likel hood of their sons exhibiting the 4) How can Akhtar share her mum’s genotype, but not the trait? Why isthis aso seen with Borte&her mum? 5) What can you infer about the location & nature of the mutation in the X2 allele (Borte& her mum 6) Design PCRRPL based diagnostic for the X4 allele in Ms Ad (cannot be based on DNA-sequencing 7) Minor How would you study the mouse homologue, wee Beastie (be brief? ayhem Ms AJ Ms. A-J Temulen Borte Genghis L-m-G Northerm GAPOH Western 西