Chat with us, powered by LiveChat Question: 3. Consider A Protein Popeye That Has Two Identical Subunits; Each Subunit Binds One Molecule Of Its Ligand Spinach (S). It Has Been Determined Experimentally That The Hill Coefficient (h) For The Popeye:Spinach Interaction Is 0.5. The Cartoon Below Depicts A Conformational Change That Occurs After Popeye Binds Spinach. A) Draw The Hill Plot For Popeye ... | Wridemy

17 Aug Question: 3. Consider A Protein Popeye That Has Two Identical Subunits; Each Subunit Binds One Molecule Of Its Ligand Spinach (S). It Has Been Determined Experimentally That The Hill Coefficient (h) For The Popeye:Spinach Interaction Is 0.5. The Cartoon Below Depicts A Conformational Change That Occurs After Popeye Binds Spinach. A) Draw The Hill Plot For Popeye …

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3. Consider a protein Popeye that has two identical subunits; each subunit binds one molecule of its ligand Spinach (S). It has been determined experimentally that the Hill coefficient (h) for the Popeye:Spinach interaction is 0.5. The cartoon below depicts a conformational change that occurs after Popeye binds Spinach. a) Draw the Hill plot for Popeye binding to Spinach in the graph below, measuring Spinach binding from 1 uM to 1 M with the following binding affinities: a Ko for high affinity site 0.1 mM, a Ko for low affinity site 10 mM, and an apparent Ko for the overall binding of allosteric system at 1 mM. the 9 1-f .2 -3 3 2 10 23 log [L] (mM) b) You introduce a mutation that eliminates allosteric communication between the two subunits of Popeye, so the affinity for Spinach doesn’t change from its initial state. Draw the expected binding curve for this mutant in the graph below, indicating concentration units on the x-axis below. Give a short answer explaining your reasoning f o.5 0 0 100 200 300 400 500 600 700

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