In-class Math Worksheet 2, BS171, Spring 2019 You perform a serial dilution of a Salmonella broth culture as depicted in the image below. The initial culture has concentration of 1 x 109 cells/ml. Use the information from this image to answer questions 5-10. Note, we are starting with a high concentration of Salmonella in the culture tube and decreasing the concentration with each dilution tube. Note that even though the volumes being transferred for diluting are in μ1 amounts, cell concentration is always reported as cells/ml. You can keep the volumes in units of ul, but the units of cell concentration remain as cells/ml. Syd =Vi Tube Tube Tube Salmonella culture 100 100-ו 5. (1.5 pts) What is the total dilution from the stock culture to Tube 3? 6. (1.5 pts) What is the concentration of Salmonella in Tube 3? 7. (1 pts) After plating 100 ul from Tube 3, how many colonies do you predict to observe growing on the LB plate? 8. (1.5 pts) What is the total dilution from the stock culture to Tube 5? 9 (1.5 pts) What is the concentration of Salmonella in Tube 5? 10. (1 pts) After plating 100 l from Tube 5, how many colonies do you predict to observe growing on the LB plate?