18 Aug Question: 1. A Substrate “A” Can Be Converted To A Product “B” By Enzyme 1. Substrate “A” Can Also Be Converted To “C” By Enzyme 2. The Kmof Enzyme 1 = 23 MM The Km Of Enzyme 2 = 76 MM The Vmax Of Enzyme 1 = 133 μmoles Of Product/minute The Vmaxof Enzyme 2 = 133 μmoles Of Product/minute (a) Draw A Rough Enzyme Kinetics [S] Vs. V0graph With The …
1. A substrate “A” can be converted to a product “B” by Enzyme 1. Substrate “A” can also be converted to “C” by Enzyme 2.
The Kmof Enzyme 1 = 23 mM
The Km of Enzyme 2 = 76 mM
The Vmax of Enzyme 1 = 133 μmoles of product/minute
The Vmaxof Enzyme 2 = 133 μmoles of product/minute
(a) Draw a rough enzyme kinetics [S] vs. v0graph with the above data. Show the curve for Enzyme 1 first and then repeat for Enzyme 2.
(b) At 23 mM substrate concentration [A], what is the velocity of Enzyme 1?
(c) At 76 mM substrate concentration [A], what is the velocity of Enzyme 2?
(d) At 23 mM substrate concentration [A], what is the velocity of Enzyme 2?
(e) You do an experiment to test these enzymes. In test-tube #1, you have enzyme 1 and provide it with 23 mM of substrate A. In test-tube #2, you have enzyme 2, and you provide it with 23 mM of substrate A.
(i) How many μmoles of “B” are made in test-tube #1 after running the reaction for 1 minute?
(ii) how many μmoles of “C” are made in test-tube 2 after running the reaction for 1 minute?
2. A substrate “A” can be converted to a product “B” by Enzyme 1. Substrate “A” can also be converted to “C” by Enzyme 2.
The Kmof Enzyme 1 = 15 mM
The Km of Enzyme 2 = 34 mM
The Vmax of Enzyme 1 = 180 μmoles of product/minute
The Vmaxof Enzyme 2 = 180 μmoles of product/minute
(b) You do an experiment to test these enzymes. In test-tube #1, you have enzyme 1 and provide it with 15 mM of substrate A. In test-tube #2, you have enzyme 2, and you provide it with 15 mM of substrate A.
(i) How many μmoles of “B” are made in test-tube #1 after running the reaction for 8 minutes?
(ii) How many μmoles of “C” are made in test-tube 2 after running the reaction for 8minutes?
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