Activity 5: Effect of Inhibltors on Enzyme Activity 5a. Record your experimental data in the table below. Time Absorbance at 450 nm (Aso (min) 5001000500 1000 500 1000l ONPG+ ONPG+No ONPG + No ONPG+ONPG ONPG + 0.510.OSS | 0043 |の1000 11 ao011の 0,00 2.0 10213| o. 118 10.005 | 0.01010.005 lo oo o 5b. On separate paper, plot the data (Time (X) vs. Absorbance (Y)), using Excel. 5c. Using the “Trend Line function, do a linear regression on each set of points. Check the “Display equation on chart” box. Write the slopes of each linear regression equation for each temperature in the chart below. In each case, the slope is equal to the rate of enzyme activity measured as the change in absorbance per minute (AAsso/min). Enzyme Activity (Asemin 500 l ONPG + No Inhibitor 1000 ul ONPG No Inhibitor 500 ul ONPG+Inhibitor X 1000 ul ONPG + Inhibitor X 500 ul ONPG + Inhibitor Y 1000 μ1ONPG + Inhibitor Y Now, use the data from the above table to plot a bar graph showing reaction rate (enzyme activity) at each of the different treatments. You may need to consult with your instructor on how to do this. 5d. 13 5e. What is your conclusion about Solution X as an inhibitor? Include whether you think it is a or non-competitive inhibitor (or neither) and why Solution x inhibiter has high ccncentraacn in a 1oon ud at 3o sec but its lao at Soul 5f. What is your conclusion about Solution Y as an inhibitor? competitive or non-competitive inhibitor (or neither) and why Include whether you think it is a somat 5g. What was the purpose of the tube that just had a high concentration of ONPG, but no inhibitors?